- Myriad Genetics (2005-2008)
- Ph.D. (2003), University of California, Riverside (Dallas Rabenstein)
- B.S. (1992), Oregon State University; Quanterra Environmental Services (1994-1997)
Dr. Spain directs the shared instrument facility within the chemistry department, which includes overseeing the day-to-day operation of the NMR, mass spectrometry, and optical facilities. This involves teaching new and ongoing students how to properly utilize the available instrumentation along with method development, maintenance, tuning, optimization, and calibration of the instrumentation. Further roles of the Director of Instrumentation include planning long term needs of the department for instrumentation acquisition and teaching courses in analytical chemistry. In addition, Dr. Spain develops and maintains the department's intranet site and shared instrument facility wiki site.
Dr. Spain's primary research interests involve using NMR and mass spectrometry to solve problems in the area of biological chemistry. NMR is a powerful analytical technique that can provide site specific binding information that other instrumentation cannot. Furthermore, NMR can be used to elucidate the exchange kinetics of a wide variety of interesting problems.
One example of binding involves phytochelatins, which are peptides that plants, algae, and certain fungi produce in response to toxic heavy metal exposure. For example, upon reaction of end-capped PC2, Ac-γ-Glu-Cys-γ-Glu-Cys-Gly-NH2, with selenite both complexed and oxidized PC2 can be observed in the 2D TOCSY NMR spectrum.
The cis/trans isomerization kinetics of proline containing peptides using NMR has been extensively studied. We found that the rate of cis/trans isomerization was greatly increased upon formation of cyclic disulfides.
This rate enhancement was verified by both NMR and molecular modeling to be caused by the formation of a hydrogen bond between the NH proton of the residue following the proline to the nitrogen of proline.
- Shi, T.; Spain, S.M.; Rabenstein, D.L. A striking periodicity of the cis/trans isomerization of proline imide bonds in cyclic disulfide-bridged peptides. Angew. Chem. Int. Ed. 2006, 45, 1433.
- Spain, S.M.; Rabenstein, D.L. Characterization of the selenotrisulfide formed by reaction of selenite with end-capped phytochelatin-2. Anal. Bioanal. Chem. 2004, 378, 1414.
- Shi, T.; Spain, S.M.; Rabenstein, D.L. Unexpectedly fast cis/trans isomerization of Xaa-Pro peptide bonds in disulfide-constrained cyclic peptides. J. Am. Chem. Soc. 2004, 126, 790.
- Spain, S.M.; Rabenstein, D.L. Characterization of the acid/base and redox chemistry of phytochelatin analogue peptides. Anal. Chem. 2003, 75, 3712.
- Rabenstein, D.L.; Shi, T.S.; Spain, S.M. Intramolecular catalysis of the cis-trans isomerization of proline peptide bonds in cyclic disulfide-containing peptides. J. Am. Chem. Soc. 2000, 122, 2401.
- "Chiral analysis, asymmetric synthesis, and chiral purification of MPC-0920," 2007.
- "Methods for the asymmetric synthesis of MPC-07869," 2007.
- "Salt of tarenflurbil for the treatment of neurodegenerative diseases and cancer," 2007.
- "Compounds for the treatment of viral infections and other diseases and conditions," 2007.