Core descriptions and equipment lists: Physiology and Cell Biology Department Core Facilities

Confocal Microscopy and Calcium Imaging Facility

The confocal microscopy suite primarily serves researchers in the physiology and cell biology department. The suite contains a variety of laser confocal microscopes. Also available within the department is a workstation to visualize organelle traffic and rapid calcium waves in cells using a high-speed Nipkow dual spinning disk confocal laser imaging system.

Location: Center for Molecular Medicine Building
Phone: (775) 784-6908

Equipment List

  • Zeiss LSM 510 Meta laser confocal microscope
  • Bio-Rad MRC 600 laser confocal microscope
  • Noran Odyssey laser confocal microscope
  • Nipkow high-speed dual spinning disk confocal laser imaging system: QLC100 high-speed confocal scan head, XR-Mega-10 ICCD camera
  • Calcium imaging workstation: high-speed Nipkow dual spinning disk confocal laser imaging system consisting of a QLC100 high-speed confocal scan head, XR-Mega-10 ICCD camera, filter sets, fiber optics, 4-channel acousto-optical tunable filter (AOTF), with the scan head mounted on a Nikon E600 FN upright physioscope with fluorescent water immersion lenses

For equipment documentation, including manuals and specifications, contact Becky Albion.


Molecular Core

The department’s molecular core facility is fully staffed and equipped with shared instrumentation that includes a quantitative real-time PCR (qRT-PCR) system, a gel imager, and four 96-well thermal cyclers. Additional instrumentation includes gel electrophoresis systems, a rocking incubator, a hybridization chamber, electroporation systems, centrifuges, refrigerators, freezers, and software for sequence analysis.

Location: Center for Molecular Medicine Building
Phone: (775) 784-6908

Equipment List

  • Applied Biosystems ABI 7300 sequence detector

For equipment documentation, including manuals and specifications, contact Becky Albion.


Histology and Immunochemistry Core

The histology and immunochemistry core provides a workstation for paraffin wax-embedding, sectioning, and staining. A microscope equipped with a z-axis encoder and drawing tube is available for histology needs as well as design-based stereological quantification of particles in tissue sections. Additional equipment available includes a motorized cryostat and vibratome, an inverted upright fluorescent microscope with a digital imaging system, and a wet laboratory with dissecting microscopes for tissue processing.

Location: Center for Molecular Medicine Building
Phone: (775) 784-6908

Equipment List

  • Rotary microtome
  • Nikon microscope with a z-axis encoder and drawing tube
  • Leica CM3050 cryostat

For equipment documentation, including manuals and specifications, contact Becky Albion.