Subhash Verma, Ph.D.

Associate Professor
Headshot of Subhash Verma
Biochemical and recombinant virus approaches to establish the role of viral proteins and DNA elements in the replication and persistence of the KSHV genome

Summary

Professional Biography

  • 2014 - present: Associate Professor, Department of Microbiology and Immunology, University of Nevada, Reno, School of Medicine, Reno, NV, USA
  • 2009 - 2014: Assistant Professor, Department of Microbiology and Immunology, University of Nevada, Reno, School of Medicine, Reno, NV, USA
  • 2007 - 2009: Research Associate, Microbiology and Virology, University of Pennsylvania, Philadelphia, PA, USA
  • 2003 - 2007: Postdoctoral Fellowship, Microbiology and Virology, University of Pennsylvania, Philadelphia, PA, USA

Research Interests

My laboratory is interested in understanding the mechanism of KSHV genome replication during reactivation as well as during de-novo infection of human cells. Kaposi's sarcoma associated herpesvirus (KSHV), also referred, as Human Herpesvirus 8 (HHV8) is tightly linked to various human malignancies including Primary Effusion Lymphomas (PELs), Kaposi's sarcoma (KS) and Multicentric Castleman's Disease (MCDs) and cause tumors predominantly in immune compromised individuals including HIV+ and patients receiving immune suppressive therapies to prevent graft rejection. KSHV induced tumors are one of the major causes of morbidity and mortality of HIV/AIDS patients.

The major research interests are defining the genetic and epigenetic factors involved in regulating viral DNA replication during latent as well as lytic phases of the viral life cycles. We use a novel, Single Molecule Analysis of the Replication DNA (SMARD), approach to localize the replication initiation (ori) and termination sites on the viral genome. We are using next generation sequencing (RNA-seq and ChIP-seq) to delineate the changes in chromatin structure required for replication initiation. Additionally, we are using various other biochemical and recombinant approaches to establish the role of viral genes in replication and persistence of the viral genome.

Current Lab Members

  • Timsy Uppal Ph.D.
  • Svetlana Khaiboullina MD Ph.D.
  • Andrew Zareie, graduate student
  • Samantha Solomon (MS-Biotech)
  • Majid Khan (MS-MD)
  • Riley Berriesford (Undergrad)
  • Jay Babu (Undergrad)
  • Sivani Reganti (Undergrad)

Past Lab Members

  • Maria McDowell
  • Shanthan Challa
  • Ruth Salas
  • Simon Pinsky
  • Sharif Ramjahn
  • Pravin Kumar Purushothaman
  • James Mcguinness
  • Jessica Chen
  • Rama Raja
  • Niraj Gandhi
  • Suhani Thakker
  • Christina Chen
  • Jennifer Labaille
  • Kayla Hiura
  • Christian Stadler
  • Roxanne Strahan
  • Roni Sarkar
  • Namrata Gupta
  • Dylan Meyers
  • Kammi Moniz
  • Prerna Dabral

Education

  • 2003: Ph.D., Biotechnology/Microbiology, Banaras Hindu University, India