This media preparator allows the rapid and gentle preparation of 1-10 liter culture medium. Precise controlling of temperature, time and pressure during the sterilization process guarantee constant high quality. The mediaclave can be used for the preparation and sterilization of numerous types of culture media, including media for C. elegans research. The wide adding port facilitates safe addition of supplements prior to dispensing. The mediaclave is connected to a peristaltic pump to fill petri dishes and vials.