We offer Illumina library preparation services using commercially available kits. We have three Illumina sequencing instruments: NextSeq 2000, NextSeq 550 and MiniSeq. Our sequencing output has a range of 8 million to 1.1 billion read/read pairs, or 2.4 to 330 gigabases. This is the equivalent of 0.6 to 110 human genome equivalents.
The Nevada Genomics Center operates a 3730 DNA Analyzer for Sanger sequencing. All of our runs include an internal plasmid control to aid in quality control.
Samples submitted by 2 p.m. Monday-Thursday are guaranteed to have data returned the following day.
Fragment analysis/genotyping/DNA fingerprinting
Fragment analysis can be performed using microsatellite or RFLP techniques. Samples are run on the 3730 DNA Analyzer, which is equipped with filters compatible with 6-FAM, VIC, NED, PET and LIZ (for size standards).
We can run plates provided as-is, or prepare your samples by diluting (if necessary) and adding size standard (LIZ 500).
We will determine the best dilution for your samples if necessary for a nominal fee.
Fragment analysis/genotyping sample submission guidelines
- Submit requests with our dnaTools LIMs.
- Email firstname.lastname@example.org with any questions related to using dnaTools.
- Ensure that your sample prep process (PCR) is working optimally and robustly. We recommend being able to dilute PCRs by at least 100-fold for optimal capillary runs.
- How to submit your samples:
- Using 8-well strip tubes:
- Label each tube with the sample number (1, 2, 3,…).
- Write the order number on the side of the first tube of the strip.
- Using 96-well plates:
- Submit a maximum of 96 samples per order if you are using plates.
- Order samples down columns. Sample 1 is A1, sample 2 is B1, etc.
- Write the order number on the plate.
- Using 8-well strip tubes:
Downloading Your Fragment Analysis/Genotyping Data
Data are provided in fsa format for download via the dnaTools server.
We offer PCR cleanup for Sanger sequencing services. Your samples will be quantified and an appropriate amount is used in an enzymatic reaction that degrades remaining PCR primers and dephosphorylates remaining dNTPs. This treated sample can then be used directly in Sanger sequencing.
We use quantification methods specific for dsDNA or RNA.
Up to 48 samples will be quantified with a Qubit fluorometer.
A plate-based assay will be used to quantify 48 or more samples. Standards are measured in triplicate and you can request multiple measurements of your samples.
Agilent TapeStation 4150 services
The Agilent TapeStation 4150 can be used to determine the size range of DNA or RNA samples. RIN numbers are reported for RNA samples. We are currently offering three types of ScreenTapes as part of our regular services. Please contact us if you are interested in the High Sensitivity versions of the DNA & RNA ScreenTapes, as well as cell-free and genomic (up to 60 kb) DNA ScreenTapes.
Samples should be 0.1 to 50 ng/ul. The dynamic size range is 35 to 1,000 bp.
Samples should be 0.1 to 50 ng/ul. The dynamic size range is 100 to 5,000 bp.
Samples should be 25 to 500 ng/ul.
QuantStudio 3 services
We have a QuantStudio 3 that can be used with 0.2 ml PCR plates.