Sanger sequencing: Common primers provided by the Nevada Genomics Center
All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F, M13(-46)R, T3, T7, and SP6. If your template contains a poly A tail and you would like to sequence through it, please let us know before hand as we can use a "poly T mix" primer pool which has given good results with poly A regions.
For our Reno clients: please stop-by the Nevada Genomics Center and pick-up your free 100uL 2uM aliquot of primer for your sequencing.
For our clients outside Reno: at no extra cost we will add one of these common sequencing primers to your sequencing samples upon request.
Please Note: there are several primers called: M13F, M13R, SP6, T7 and T3. Because of this you should be sure the primer sequence listed below is homologous to your vector’s priming site.
Please check these primer sequences against your vector sequence before you have us use them on your samples!
Please see the guidelines on Sanger Sequencing: Optimal Amount of Template and Primer.