Services, instrumentation and equipment
This page contains links to third party online content. If you experience any issues accessing this content, please contact the Nevada Proteomics Center.
Mass spectrometry services
We offer mass spectrometry services such as:
- Gel, IP and solution based protein identification
- Proteome quantitation using both isobaric labelling (TMT) and label free methodologies
- Post-translational modification analysis
- Sample preparation (if you are preparing samples yourself, please review our sample prep information)
Mass spectrometry instrumentation and sample prep equipment
Visit Nevada INBRE for information about grant citations. In addition, samples analyzed using either the Bruker timsTOF Pro2 or the Evosep One LC should also acknowledge the National Institutes of Health Shared Instrumentation Program (1S10OD028530-01A1).
The Eclipse will allow researchers the ability to obtain insight into the most complex biological systems from whole proteome profiling and quantitation, structure characterization and multiplex proteomics. The Eclipse is capable of performing a multitude of dissociation techniques, including CID, HCD, ETC, EThcD, and UVPD, making it the most powerful system available for comprehensive characterization of proteins. Proteome characterization using MS3 -based Tandem Mass Tag (TMT) method, enhanced by Real-Time Search, provides the throughput and sensitivity to achieve in-depth proteome coverage and quantitative accuracy. Further, using the novel Thermo Scientific TMTpro16plex label reagent set, up to 16 single samples can be analyzed in one LC-MS run, providing quantitative comparison of thousands of proteins among individual cells.
The timsTOF Pro 2 uses the parallel accumulation serial fragmentation (PASEF®) acquisition method to provide extremely high speed and sensitivity, requiring only minimal sample amounts providing 4D-Proteomics allowing researchers to reach greater depths in proteomics. Trapped ion mobility spectrometry (TIMS) is first and foremost a separation technique in the gas phase. TIMS separation increases selectivity, excludes singly charged precursors from fragmentation and cleans up the sample by concentrating signals from noise. dia-PASEF is both more sensitive and selective than traditional DIA approaches as it applies the PASEF principle to data independent acquisition, combining the advantages of DIA with the inherent ion efficiency of PASEF.
The Evosep One liquid chromatography system is designed to provide clinical diagnostics through protein-based analysis. The Evosep One coupled to either the Thermo Eclipse or Bruker timsTOF provides researchers a robust high-throughput platform to quickly and efficiently compare biological samples, such as blood or biopsies, in order to deliver a diagnosis/verdict of healthy or diseased.