|Contact Information for Nevada Proteomics Center|
|Phone||(775) 784-6337: 2-D Gels|
|Phone||(775) 784-1590: Mass Spectrometry|
|Location||School of Medicine
Manville Medical Building, Rooms 15-17, MS 0330
|Address||1664 N. Virginia Street
Reno, NV 89557-MS 0330
The Mass Spectrometry Lab of the Nevada Proteomics Center offers a variety of services. These include enzymatic protein digestion, LC/MSMS, MALDI/MSMS, and GC/MS.
Before being analyzed by mass spec, most proteins must be broken into smaller fragments. This is accomplished by digestion with a protease, usually trypsin, but may be another proteases of your choice.
Requirements: >100 fmol protein, may be a protein in solution, a gel slice or spot that is visible by coomassie staining.
Requirements: >50 fmol of tryptic digest on column; sample in an aqueous solution of <5% acetonitrile, <0.01% (w/v) detergent concentration.
MALDI analysis is performed using an Applied Biosystems 4700 Proteomics Analyzer with TOF/TOF Optics. MALDI services include Protein Identification and accurate mass determination.
Requirements: Protein Identification - visible spots by CBB staining (>100 fmol); accurate mass - >10 fmol; average mass >1 pmol protein and in appropriate solution.
GC/MSn analysis is performed using a Thermo Finnigan Polaris Q. The GC/MS is used primarily for analysis of small nonpolar volatiles.
Requirements: sample in nonpolar solvent, >10 ng.