|Colony of Bombus impatiens, Koppert Biological||$257.00||4||$1028.00|
|Shipping on Bumblebee Colonies (overnight MI to NV)||$75.00||4||$300.00|
|Pollen for 4 colonies for 3 months||$6/lb.||12lbs||$72.00|
|5g Octopamine hydrochloride, Sigma Aldrich||$98.82||1||$98.82|
Bumblebee colonies will be obtained from Koppert Biological Systems (MI, USA) and shipped overnight to Nevada. An emerging standard for publication is the utilization of three to five colonies for testing so as to minimize individual colony biases and to acquire a sufficient data set. In order to mainta in the colonies I will need pollen to feed them on a daily basis. Additionally I will use the chemical octopamine hydrochloride to explore neuroendocrine relationships between gustatory responsiveness, learning, and octopamine.
|LIVE/DEAD® Sperm Viability Kit||Quantification of sperm number and viability||ThermoFisher Scientific/L7011||$407.00|
|Avant Guard 20ul Barrier tips||For processing sperm samples||MidSci/AV20||$66.00|
|Plastic vials (1,000)||Individual rearing of study organism||BioQuip/8909||$175.00|
|Drosophilia culture vials||Rearing prey fruit flies||Carolina Biological Supply/173080||$150/00|
|Formula 4-24®Instant Drosophilia Medium, Blue, Case of Four 4-L Bags||Rearing prey fruit flies||Carolina Biological Supply/173214||$85.95|
|Microflex Latex Evolution One Gloves, Small (Pack of 1000)||Amazon/EV-2050-S||$128.00|
|PureLink miRNA isolation kit||Small RNA extraction||ThermoFisher Scientific /K157001||$211.00|
|PureLink® RNA Mini Kit||Large RNA extraction||ThermoFisher Scientific/12183018A||$252.00|
|Equipment, Justification, Product Website URL||Cost|
|Hall Effect Sensor (x64)These provide position data for the program to interpret.
|Magnets (x32)These will be embedded into the bottom of the pieces.
|3D printed pieces*This is the cost for printing the chess pieces.||$300|
|Eight-channel 16bit A/D converter (x8)This handles the data from 8 hall effect sensors attached to each Arduino Shield.
|Arduino Shields (x8)The sensors are attached to these boards in a specific pattern, forming an array.
|Arduino Uno (x1)This micro-computer interprets the sensor data and handles the audio files.
|Cycling 74' MAXThis is the computer code language used for the program. (Cost is estimated for 6 months of use.)
|Misc. Electronic components*This includes the solder, wires, connectors, and other items to build the sensor array.||$100.00|
|Chessboard*This is the playing surface for the game, as well as the element that the sensor array is attached to.||$30.00|
|Wood sheets (x4)*These will be used to build the pillar housing the board, electronics, and speakers.||$58.00|
|Chairs (x2)*Simple chairs for players to sit and play the game.||$30.00|
|Speakers (x2)*These will play the audio files triggered by the player actions.
|Misc. ExpensesThis funding is for unexpected expenses.||$60|
I estimated shipping costs to the best of my knowledge.
* Costs are estimated based on average costs of the material; final cost may be slightly different.