- Microinjection: The injection of embryonic stem (ES) cells into mouse blastocysts to create chimeric mice
- Pronuclear Injection: The injection of isolated construct DNA into the pronucleus of fertilized mouse embryos to create transgenic mice
- Cryopreservation: The freezing and storage of desired sperm and eggs in liquid nitrogen
- Cryorecovery: The recovery of viable frozen sperm and eggs
- Genotyping: The screening of DNA for specific genes by PCR
- Chromosome counting: The isolation and counting of chromosomes of a desired specimen
- In vitro fertilization: The fertilization of eggs by sperm in culture
- Breeding mice: The expansion and maintenance of a particular strain
- Rederivation: The process in which embryos are harvested and transferred into a pseudopregnant clean surrogate mother in a clean facility to establish a ‘clean' strain
- ES cell gene targeting: Adding an isolated DNA construct into the genome of mouse embryonic stem (ES) cells by electroporation
- Assay ES cell clones by PCR/Southern blot: Screening embryonic stem (ES) cells by either PCR or Southern blot for a specific gene
- Target construction: The design and creation of a specific segment of DNA
The Nevada Transgenic Center provides the services to maintain mouse colonies for investigators, genetic following of colonies, and can suggest breeding strategies to prevent genetic drift in colonies.
Please contact the Department of Lab Animal Medicine at (775) 784-4874 for pricing information.