|Contact Information for Nevada Proteomics Center|
|Phone||(775) 784-6337: 2-D Gels|
|Phone||(775) 784-1590: Mass Spectrometry|
|Location||School of Medicine
Manville Medical Building, Rooms 15-17, MS 0330
|Address||1664 N. Virginia Street
Reno, NV 89557-MS 0330
The GPS Mascot report has a list of each spot. Under each spot is a list of the top protein hits based on MOWSE score, a number assigned by number of fragments submitted and search parameters assigned including size of database. You can find the math theory behind the score at Matrix Science. The report also lists name of protein, accession number from the database, protein score, confidence interval, and ion score.
The Well position names have not been conserved from the MTP to the MALDI target. The PDQuest spot numbers can be found at the far left under each gray header bar.
The other Excel file we attach lists what the digestion spectrum looked like i.e. good, low peptide signal. These should help you determine if your MASCOT score is low because it didn’t find a match in the database or if there was not enough raw data available for a high score (these would be the low peptide signal samples).
We can also send other varieties of the MASCOT report. If you would like to see the matching peptide list for any or all of your spots please let us know. Our goal is to not overwhelm you with data if you don’t know what you are looking at.
he Applied Biosystems software package has its own interface for viewing the MASCOT results. At this time, the content of the data exported as excel reports cannot be modified. Not satisfied with that answer? Ask us about viewing your data on IDQuest.
A short description of the protein digestion/MALDI analysis can be found here. If you want a more detailed protocol or if your samples were analyzed by a different method, please contact us and we will be happy to send you the required information.