|Contact Information for Nevada Proteomics Center|
|Phone||(775) 784-6337: 2-D Gels|
|Phone||(775) 784-1590: Mass Spectrometry|
|Location||School of Medicine
Manville Medical Building, Rooms 15-17, MS 0330
|Address||1664 N. Virginia Street
Reno, NV 89557-MS 0330
The standard method for maintaining reduced cysteines during the first dimension is to add reducing agents, such as DTT or TBP (tributyl phosphine), or disulfide exchange reagents, such as DeStreak, to the rehydration buffer. However, streaking in some samples is significantly reduced if cysteines are both reduced and alkylated before the first dimension using the methods below.