Institutional Development Award
|Contact Information for Nevada Proteomics Center|
|Phone||(775) 784-6337: 2-D Gels|
|Phone||(775) 784-1590: Mass Spectrometry|
|Location||School of Medicine
Manville Medical Building, Rooms 15-17, MS 0330
|Address||1664 N. Virginia Street
Reno, NV 89557-MS 0330
Protocol from Promega:
Note: PNGase F cannot cleave when an α1-3 Fucose is attached to the core GlcNAc.
New England BioLabs has a large variety of glycosidases for use in removing or characterizing glycans.
QA Bio has a number of kits that will remove glycans from proteins.
QA Bio Catalog No.: KE-DGMX or KE-DG01
Completely removes all N-linked glycans and most O-linked glycans.
QA Bio Catalog No.: LL-ORELA-A2
Chemically releases O-glycans, then fluorescently labels the Ser or Thr to which the glycans were attached.
QA Bio Catalog No.: KE-EFX3)
Cleaves after the N-acetyl glucosamine attached to Asn on a variety of N-linked glycans. A GlcNAc remains attached at the location of polysaccharide chain.