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Nevada Proteomics Center

Phone(775) 784-6337: 2-D Gels
Phone(775) 784-1590: Mass Spectrometry
Fax(775) 784-1419
Emailquilici@unr.edu
Location School of Medicine
Manville Medical Building, Rooms 15-17, MS 0330
Address 1664 N. Virginia Street
Reno,  NV  89557-MS 0330
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EZQ Protein Assay

The EZQ Protein Assay from Invitrogen is used to assay the protein concentration of samples in rehydration buffer just before they are applied to IPG strips. The EZQ method is a fluorescent assay that is very sensitive, uses uses only 4 µl of sample and is not affected by the presence of detergents, reducing agents or chaotropes. Knowledge of the protein concentration in the sample allows us to apply the optimum amount of protein for the best 2-D gel results and to normalize the amount of protein applied for each member of a set of samples.

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University of Nevada, Reno

University of Nevada, Reno
1664 N. Virginia Street
Reno,  NV  89557-

(775) 784-1110
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