Cynthia Mastick, Ph.D.
- B.Sc. Biochemistry, 1985 Carnegie Mellon University, Pittsburgh, PA
- Ph.D. Cellular and Molecular Biology, 1990 Carnegie Mellon University, Pittsburgh, PA
The main role of insulin in the body is to regulate the levels of glucose and lipid in the blood. After feeding, when glucose and lipid levels are high, insulin is secreted from the pancreas and travels through the blood to three major target tissues: liver, muscle, and adipose tissue (fat). In these tissues, insulin stimulates the clearance of glucose and lipid from the blood by stimulating the conversion of these molecules into glycogen and fat, and preventing the breakdown of these storage molecules. Insulin also increases the rate of glucose transport into fat and muscle (10-50-fold). In type II, or adult onset diabetes, the target tissues no longer respond to insulin. This results in elevated blood glucose and lipid levels, which leads to the major complications of this chronic and debilitating disease. These include heart disease, kidney failure, blindness, and nerve damage (resulting in amputation). The main research questions in my lab are how does insulin regulate glucose transport and glucose metabolism at the molecular level, and what goes wrong with these pathways in type II diabetes. Our goal is to identify novel therapeutic targets to treat this very common, devastating disease. Current research is focused on the regulation of glucose transport in adipocytes.
- Cao, H, WE Courchesne, and CC Mastick (2002) A Phosphotyrosine-Dependent Dihybrid Yeast Protein Interaction Screen Reveals a Role for Phosphorylation of Caveolin-1 on Tyrosine 14: Recruitment of CSK to Caveolae. Accelerated Publication, J. Biol. Chem., 277:8771-8774. http://www.jbc.org/cgi/content/full/277/11/8771
- Sanguinetti, A.R. and C.C. Mastick (2003) cAbl Expression is Required for Oxidative Stress-Induced Phosphorylation of Caveolin-1 on Tyrosine 14. Cellular Signaling, 15:289-298. doi:10.1016/S0898-6568(02)00090-6
- Muretta, J., Romenskiai, I., Cassiday, P. A., and Mastick, C. C. (2007) Expression of a synapsin IIb site 1 phosphorylation mutant in 3T3-L1 adipocytes inhibits basal intracellular retention of Glut4. J. Cell Sci. 120:1168-77. http://jcs.biologists.org/cgi/content/full/120/7/e702
- Muretta, J., Romenskiai, I., CC Mastick (2008) Insulin Releases Glut4 from Static Storage Compartments into Cycling Endosomes and Increases the Rate Constant for Glut4 Exocytosis. J. Biol. Chem., 283: 311-23. http://www.jbc.org/cgi/content/full/283/1/311
- Muretta, JM, Mastick, CC (2009) How Insulin Regulates Glucose Transport in Adipocytes. Vitamins and Hormones, Special Issue on Insulin and IGFs, Vol. 80: 241-282. doi:10.1016/S0083-6729(08)00610-9