Scholar: Arlette Lunoz
Faculty Mentors: Dr. Suk-Wah Tam Chang
Research Topic: Quenching of Fluorescence Emission of TAMRA-labeled DNA Oligonucleotides by Gold Nanoparticles: A Study of the Effects of Oligomer Length and Sequence
Abstract: The quenching of fluorescence emission from fluorophore-labeled oligonucleotides by gold nanoparticles has been reported in the literature as a mechanism for sequence specific detection of DNA targets. In this project, we aim to learn more about the effects of the oligonucleotide sequences and the length of the oligonucleotides that separate the fluorophore from the gold nanoparticle on the quenching efficiency. This knowledge would be useful for future designs of effective quenching systems for DNA sensing. TAMRA-labeled oligonucleotides of various lengths (from 19mers to 49mers) were designed and custom synthesized. Their complementary sequences were functionalized with disulfide linkers and were attached to gold nanoparticles via the disulfide group. The change in fluorescence emission intensity of TAMRA-labeled oligonucleotides upon hybridization with their complementary oligonucleotides on gold nanoparticles was monitored.
Earned Baccalaureate Degree: Spring 2011
Doctoral Program Update: Accepted into Oregon State University Pharmacology Program for fall 2011.