David A. Schooley
Department: Biochemistry and Molecular Biology
Academic Unit: College of Agriculture, Biotechnology and Natural Resources
Professional degrees (Degree, year, Institution):Ph.D., Stanford University, 1968
Mail Stop: 330
Insect Biochemistry and Physiology; Biochemistry
We have studied insect juvenile hormone biochemistry extensively, and identified
four of the six known insect juvenile hormones and a similar molecule from
crabs. We have investigated the biosynthesis and metabolism of the juvenile
hormones, showing that the "extra" carbons in the homosesquiterpenoids
comes from propionate. The endocrine glands that make these hormones derive
propionate from the amino acids valine and/or isoleucine.
Most life functions in insects are controlled by peptide hormones. Peptides primarily affect the activity of existing enzymes in the cell, a very rapid action. In the last two decades we isolated and identified a variety of important insect peptide hormones. Recently we have concentrated on hormones affecting water balance, and identified 11 diuretic hormones from seven insect species, and also discovered two antidiuretic peptides which decrease urine production. We used these hormone sequences to find similar hormones in the genomes of insect species including Drosophila and the malaria mosquito Anopheles. We synthesized these peptides and collaborated with insect physiologists to understand how these hormones control urine production. We have determined three dimensional structures of several of these hormones by molecular modeling and spectroscopic analysis, and have used differential proteomics to study the mechanism of signal transduction of a diuretic hormone in the target tissue. Ultimately this knowledge may turned against insects, creating new, selective pesticides which are fast acting.
Current Graduate Students
Other Lab Members
Lilly Ng, part-time
J R Floriani, Sr. Thesis
Vasopressin-like peptide and its receptor function in an indirect diuretic
signaling pathway in the red flour beetle, M.J. Aikins, D.A. Schooley, K.
Begum, M. Detheux, R.W. Beeman, and Y. Park, Insect Biochem. Mol. Biol.
38, 740-748 (2008).
Amino acid sequence and biological activity of a calcitonin-like diuretic
hormone (DH31) from Rhodnius prolixus. V. A. Te Brugge, D. A. Schooley, and
I. Orchard, J. Exp. Biol., 211, 382-390 (2008).
An antidiuretic peptide (Tenmo-ADFb) with kinin-like diuretic activity on
Malpighian tubules of the house cricket, Acheta domesticus (L.). G. M. Coast,
R. Nachman, and D. A. Schooley, J. Exp. Biol., 210, 3979-3989 (2007).
Central peptidergic ensembles associated with organization of an innate behavior,
Y.-J. Kim, D. Zitnan, K.-H. Cho, D. A. Schooley, A. Mizoguchi, and M. E. Adams,
Proc. Natl. Acad. Sci. USA, 103, 14211-14216 (2006).
Juvenile hormone diol kinase, a calcium-binding protein with kinase activity,
from the silkworm, Bombyx mori, S. Li, Q.-R. Zhang, W.H. Xu, and D. A. Schooley,
Insect Biochem. Mol. Biol., 35, 1235-1248 (2005).
Faculty by research area
- Mastick, C
- Mastick C.
- Mastick G.
- Van der Linden
- von Bartheld
- von Bartheld