Josh E. Baker, Ph.D.
Department: Biochemistry and Molecular Biology
Academic Unit: School of Medicine
Title: Assistant Professor
Research Area: Biochemistry
Mail Stop: 330
Phone: (775) 784-4631
Fax: (775) 784-1650
We are interested in understanding the molecular basis for cell motility. This is a highly interdisciplinary problem that involves determining how molecular motors in cells convert the chemical energy from ATP hydrolysis into mechanical work. Because the chemical mechanisms of these biological motors are fundamentally different from most motor mechanisms devised by humans, these studies have clear implications for nanotechnology as well as biology.
Our current focus is on studying cooperative interactions among myosin motors in cells. Interestingly, myosin molecules function as force sensors as well as force generators, and so when many myosin molecules function together in ensemble systems like muscle, the force generated by one myosin can cooperative affect the biochemistry of neighboring myosin molecules. To better understand the interplay between myosin’s force generating and force sensing biochemistry, we use state-of-the-art experimental techniques (laser traps, fluorescence microscopy, and single molecule imaging) to measure and systematically perturb (chemically, genetically, and biologically) the mechanics and chemistry of both single myosin motors and collective motor systems. We integrate these results into mathematical and computer models in an effort to better understand the molecular basis for cell motile processes ranging from muscle contraction to intracellular transport.
Current Graduate Students:
Selected Publications (5 would be ideal; links to open access publications can be created):
Baker JE, Krementsova EB, Kennedy GG, Armstrong A, Trybus KM, Warshaw DM. Myosin V processivity: multiple kinetic pathways for head-to-head coordination. Proc. Natl. Acad. Sci. USA. 101, 5542-6 (2004).
Baker JE. Free energy transduction in a chemical motor model. J. Theor Biol. 228, 467-76 (2004).
Baker, JE, Brosseau, C, Fagnant, P, Warshaw, DM. The unique properties of tonic smooth muscle emerge from intrinsic as well as intermolecular behaviors of myosin molecules. J. Biol Chem. 278, 28533-9 (2003).
Baker JE, Brosseau C, Joel PB, Warshaw DM. The biochemical kinetics underlying actin movement generated by one and many skeletal muscle myosin molecules. Biophys. J. 82, 2134-47 (2002).
Faculty by research area
- Mastick, C
- Mastick C.
- Mastick G.
- Van der Linden
- von Bartheld
- von Bartheld