Proteomics

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Facilities Description
2-D Gels
Mass Spectrometry
Edman Protein Sequencing
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Investigators using INBRE facilities, services or resources are required to cite NIH Grant Number P20 RR-016464 from the INBRE Program of the National Center for Research Resources in all publications and presentations. © Nevada INBRE 2006.

Instrumentation - Sample Identification

	Applied Biosystems 4700 Proteomics Analyzer Matrix Assisted Laser Desorption Ionization Time of Flight/Time of Flight (MALDI TOF/TOF) mass spectrometer Linear and reflector modes
	Michrom Paradigm MS4 Multi-Dimensional Separation Equipped with autosampler for high throughput LC/MS and 2D LC/MS
	ThermoFinnigan LCQ Deca XP+ (Interfaced to above Michrom Paradigm)
	ThermoFinnigan Polaris Q GC-MS Volatile and small non-polar metabolite analysis MS/MS capability EI and CI ionization
	ABI 492 Procise Edman Protein Sequencer Gold standard for unamiguous protein sequence analysis Requires 5-10 pmol of sample as minimum quantity Can identify about 2 amino acids per hour