Proteomics






Investigators using INBRE facilities, services or resources are required to cite NIH Grant Number P20 RR-016464 from the INBRE Program of the National Center for Research Resources in all publications and presentations. © Nevada INBRE 2006.
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2-D Gel Services

 

NevadaProteomicsCenter2-DGelsLabThe 2-D gel lab of the Nevada Proteomics Core Facility is a dedicated 522 square foot facility containing all equipment necessary for sample preparation, running of 1-D or 2-D gels, staining and imaging of gels, computer analysis and comparison of gel patterns and cutting of spots.

 

The laboratory also contains all equipment and supplies necessary for most sample preparation and clean-up techniques used for bacterial, fungal, plant, insect and mammalian samples.

 

Customers are encouraged to consult with the laboratory staff before initiating proteomics experiments.  We are always glad to advise customers as to the most fruitful approaches in order to gain the desired information and to share sample preparation protocols.

 

Our equipment, which has been purchased from Bio-Rad (Hercules, CA), includes:

  • 2 Protean IEF cells for isoelectric focusing of 1-12 IPG strips from 7 to 24 cm
  • 2 Criterion electrophoresis cells capable of running 1-2 or 3-12 Criterion (13.3 x 8.7 cm) gels
  • 2 Protean II cells capable of running 1-2 or 3-6 Protean II XL (18.3 x 19.3 cm) gels
  • 1 Protean Plus cell capable of running 1-12 Protean Plus (25.6 x 23.0 cm) gels
  • MicroRotophor for in-solution isoelectric focusing
  • Trans-Blot Electrophoretic Transfer Cell for blotting of 3 Protean Plus, 3 Protean II or 12 Criterion gel