Central Services Core






Investigators using INBRE facilities, services or resources are required to cite NIH Grant Number P20 RR-016464 from the INBRE Program of the National Center for Research Resources in all publications and presentations. © Nevada INBRE 2006.
National Center for Research Resources Nevada INBRE National Institutes of Health

Pricing

 

You will be charged for all requested procedures regardless of their final outcome. Failed reactions resulting from instrument malfunction will be repeated at no additional charge. Please see additional pricing notes below. Prices effective 7/1/07.

 

Services

Per

NSHE users

non-NSHE users

Sequencing reaction & 3730 run, full plate (95 samples in a PCR plate with correct amount of DNA)*

plate

$247.00

$363.24

Sequencing reaction & 3730 run, individual samples
(samples in 200ul 8-strip tubes with correct amount of DNA) **

sample

$3.52

$5.18

Sequencing reaction setup through 3730 run
(sample submission is incorrect) †

sample

$4.60

$6.77

BigDye for BAC, cosmid or fosmid sequencing reactions

sample

$2.03

$2.03

DNA: normalize concentrations

sample

$1.48

$2.13

DNA/RNA quantitation

sample

$0.57

$0.83

Fragment Analysis: 3730 run only

sample

$0.69

$1.01

Fragment Analysis: add size std & 3730 run

sample

$0.92

$1.33

Fragment Analysis: dilute 1 PCR rxn, add size std & 3730 run

sample

$1.23

$1.77

Fragment Analysis: multiplexing/diluting (per dye)

sample

$0.31

$0.44

PCR cleanup & quantitation

sample

$1.60

$2.36

PCR 10ul reaction

sample

$0.78

$1.13

PCR 10ul multiplex reaction

sample

$0.87

$1.27

PCR 15ul reaction

sample

$0.92

$1.35

PCR 15ul multiplex reaction

sample

$1.05

$1.55

Plasmid prep, full plate

plate

$154.03

$226.52

plasmid prep, seq-3730, full plate, from cell pellets

plate

$403.64

$593.59

Affymetrix chip processing, 1-cycle (you provide chip)

chip

$312.96

$460.24

Affymetrix chip processing, 2-cycle (you provide chip)

chip

$396.37

$582.90

Agilent RNA analysis (12 samples/chip)

chip

$63.47

$93.33

Real Time PCR, Instrument use only

run

$25.22

$37.09

NSHE is the Nevada System of Higher Education

 

Pricing Notes

*Sequencing reactions through 3730 run - per plate

This price applies only to plates which have the correct concentration of DNA and primer (if custom) aliquoted into the wells of a 96-well PCR plate.  We can dry down the whole plate and resuspend in the correct volume if your samples are too dilute to send 5ul.  All other samples will be treated as “per sample”, even if sent in lots of 96.

 

**Sequencing reactions through 3730 run - per sample

This price applies only to samples which have the correct concentration of DNA and primer (if custom) aliquoted into 200ul strip tubes. We can dry down the samples and resuspend in the correct volume if your samples are too dilute to send 5ul.  All other samples will include a 'reaction setup/handling' fee

 

†Sequencing reaction setup through 3730 run

This price applies to samples that do not have the correct concentration of DNA and primer, or samples that are not in 200ul strip tubes.  The 'reaction setup/handling' fee will apply to any samples that are sent in 200ul individual tubes, 500ul tubes or 1.5ml tubes, to any DNA samples that we must dilute or otherwise aliquot and to any custom primers that need dilution or need to be added by us to your DNA samples.

 

Large scale sequencing projects

Please call for pricing if you have an EST or other large scale (>20 plates) project.