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Phone:  (775) 784-4824 
Fax: (775) 784-1620

 

Research Interests:

My laboratory is working on the molecular mechanism of herpesvirus DNA replication.  We are currently working with two herpesviruses.  The first, human cytomegalovirus (HCMV) is a large DNA virus with one known origin of DNA replication (oriLyt).  Our interest is in the initial events in viral DNA synthesis.  To this end, we are constructing mutant viruses in which a specific replication gene is deleted.  This new mutant virus is then used to infect human cells to observe how the virus replicates with an essential gene missing from the genome.

The second virus we work with is a recently discovered human herpesvirus, human herpesvirus type 8 (HHV-8) also known as kaposi’s sarcoma associated herpesvirus (KSHV).  This virus primarily infects B-cells where it is latent until stimulated to under go lytic replication in cell culture.  Our current focus is to elucidate the cis and trans acting factors required for lytic DNA replication.

Nuclear localization of UL84-EGFP and IE2 in transfected/infected HFF cells.HFF cells were transfected with pUL84-EGFP and subsequently infected with HCMV.  Cells were reacted with antibody specific for IE2 and visualized by IFA.  UL84-EGFP fusion protein was visualized by direct fluorescence microscopy at 24-120 hpi.  Arrows indicate the colocalization of UL84 and IE2 (yellow) at 48, 72, 96 and 120 hpi.

Selected Publications:

Deyde V, Rizvanov A, Otteson E, Brandt S, Bego M, Pari G, Kozel T and St Jeor S. 2005. Identification of a monoclonal antibody from Peromyscus maniculatus (deer mouse) cytomegalovirus (PCMV) which binds to a protein with homology to the human CMV matrix protein HCMV pp71. Journal of Virological Methods. 123(1):9-15

Colletti, K.S., Y. Xu,, Yamboliev, I. and G. S. Pari. 2005. Human Cytomegalovirus UL84 Is a Phosphoprotein That Exhibits UTPase Activity And Is A Putative Member Of The DExD/H Box Family Of Proteins. Journal of Biological Chemistry. 280:12. 11955-11960.

Xu, Y., AuCoin, D.P.., Huete, A.R., Cei, S.A., Hanson, L.J. and G. S. Pari. 2005. A Kaposi’s Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 ORF50 deletion mutant is defective for reactivation of latent virus and DNA replication. Journal of Virology. 79:3479-3487.

Bego, M., Maciejewski, J., Khaiboullina, S., Pari, G. and S. St Jeor. 2005. Novel Cytomegalovirus transcript present in monocytes of a latently infected HCMV donor. 79: 11022-11034. Journal of Virology.

Xu, Y., Rodriguez-Huete and G. S. Pari. 2006. Evaluation of Kaposi’s sarcoma-associated herpesvirus/human herpesvirus 8 lytic origins of replication in the context of the viral genome. 80:9905-9909. Journal of Virology.

Colletti, K., Smallenburg, K. and G.S. Pari. 2007. Human Cytomegalovirus UL84 interacts with an RNA stemloop sequence found within the RNA/DNA hybrid region of oriLyt. 81:7077-7085. Journal of Virology.

Kato-Noah, T., Rossetto, C., Xu, Y., Colletti, K., Yamboliev, I., Papousková, I. and G.S. Pari. 2007. Over-Expression Of The Kaposi’s Sarcoma-Associated Herpesvirus Transactivator K-Rta can complement a K-bZIP deletion BACmid and yields an enhanced growth phenotype. Journal of Virology. 81: 13519-13532.

Rossetto, C., Gao, Y., Yamboliev, I., Papousková, I., and G. S. Pari. 2007. Transcriptional repression of K-Rta by Kaposi's sarcoma-associated herpesvirus K-bZIP is not required for oriLyt-dependent DNA replication. 369:340-350. Virology.

Gao, Y., Colletti, K. and G. S. Pari. 2008. Identification of Human Cytomegalovirus UL84 viral and cellular encoded binding partners using Proteomics Analysis. 82: 96-104. Journal of Virology.