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Research Interests:
In Utero Gene Therapy and Stem Cell Transplantation.
My research interests are focused on developing methods of safely and efficiently performing gene therapy before to birth, thus allowing correction of genetic disorders prior to the onset of disease and resultant tissue damage. Approaches being explored are systems to allow targeting of specific cell types following direct administration of viral-based gene delivery vectors to the fetus and using genetically modified hematopoietic stem cells as gene delivery vehicles. These technologies are then being tested in a preclinical model of the bleeding disorder Hemophilia A.
Education:
B.A., Molecular Biology, 1991, Colgate University
Ph.D., Cellular & Molecular Pharmacology and Physiology, 1998, University of Nevada, Reno
Major academic interests:
In Utero Gene Therapy and In Utero Stem Cell Transplantation
Representative publications:
1. Porada, C.D., Park, P.J., Almeida-Porada, G., Glimp, H.A., Zanjani, E.D. Male germline cells are at risk following direct injection gene therapy in utero. (manuscript submitted to Molecular Therapy, 2005).
2. Porada, C.D., Park, P.J., Almeida-Porada, G., Liu, W., Ozturk, F., Glimp, H.A., Zanjani, E.D. Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer, Molecular Therapy, 11(2):284-293, 2005.
3. Almeida-Porada G., Porada, C.D., Torabi A., Zanjani, E.D. Formation of human hepatocytes by human hematopoietic stem cells in sheep. Blood 104(8):2582-90, 2004.
4. Almeida-Porada, G., Porada, C., Zajani, E.D. Plasticity of Human Stem Cells in the Fetal Sheep Model of Human Stem Cell Transplantation. Int’l J. Hem. 79:1-6, 2004.
5. Porada C.D., Park P., Almeida-Porada G., Zanjani E.D. The sheep model of in utero gene therapy. Fetal Diagn Ther. 19(1):23-30, 2004.
6. Porada C.D., Tran N.D., Almeida-Porada G., Glimp H.A. Pixley, J.S. Zhao Y., Anderson W.F., Zanjani E.D. Transduction of Long-Term Engrafting Human Hematopoietic Stem Cells by Retroviral Vectors. Human Gene Therapy 13(7):867-79, 2002
7. Tsai E., Kirby M.R., Hsu A.P., Seidel N.E., Porada C.D., Malech H.L., Zanjani E.D., Bodine D.M., Puck J.M. Retroviral transduction of IL2RG into CD34+ cells from XSCID patients permits human T and B cell development in sheep chimeras. Blood 100(1):72-9, 2002
8. Tran, N.D., Porada, C.D. (co-first author), Almeida-Porada, G., Glimp, H.A., Anderson, W.F., Zanjani, E.D. Induction of Stable Prenatal Tolerance To ?-Galactosidase By In Utero Gene Transfer Into Pre-Immune Sheep Fetuses. Blood 97:3417-3423, 2001.
9. Tran N.D., Porada C.D., Zhao Y., Almeida-Porada G., Anderson W.F., Zanjani E.D. In utero transfer and expression of exogenous genes in sheep. Experimental Hematology 28:17-30, 2000
10. Porada, C.D., Tran N., Zhao, Y, Anderson, W.F., Zanjani, E.D. Neonatal Gene Therapy: Transfer and Expression of Exogenous Genes In Neonatal Sheep Following Direct Injection of Retroviral Vectors Into The Bone Marrow Space. Experimental Hematology 28:642-650, 2000
11. Porada C.D., Tran N., Moen R., Troutman L., Flake A.W., Eglitis M., Anderson W.F., Zanjani E.D. In utero gene therapy: Transfer and long-term expression of the bacterial NeoR gene in sheep following direct injection of retroviral vectors into pre-immune fetuses. Human Gene Therapy 9:1571-85, 1998
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